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KMID : 0665220230360040244
Korean Journal of Food and Nutrition
2023 Volume.36 No. 4 p.244 ~ p.252
Antioxidant Activity and Anti-Obesity Effects of Mixture of Brassica juncea and Black Rice Bran
Im Ji-Heon

Lim June-Seok
Han Xionggao
Men Xiao
Oh Geon
Cho Geun-Hee
Choi Sun-Il
Hwang Woon-Sang
Lee Ok-Hwan
Abstract
This study was conducted to provide basic data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS) production of a mixture of Brassica juncea extract (BJE) and fermented black rice fraction (BRF). We investigated the total phenol content, total flavonoid content, antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay) and anti-obesity activity of the mixture in 3T3-L1 cells. Our results showed that the total phenol and flavonoid content increased with increasing BRF mixture ratio. The antioxidant activity increased as the BRF mixture ratio increased. In addition, BJE and BRF mixtures did not show any cytotoxicity during the 3T3-L1 differentiation period. During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS production compared to the control group. These results warrant further experiments to develop an anti-obesity functional food using a mixture of BJE and BRF.
KEYWORD
Brassica juncea, black rice bran, mixture, antioxidant, anti-obesity
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